tissue cryopreservation solution (Miltenyi Biotec)
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tissue cryopreservation solution
Tissue Cryopreservation Solution, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/129/MACS+Freezing+Solution/pm42885237-252-16-22
Average 93 stars, based on 9 article reviews
Tissue Cryopreservation Solution, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/129/MACS+Freezing+Solution/pm42885237-252-16-22
Average 93 stars, based on 9 article reviews
tissue cryopreservation solution - by Bioz Stars,
2026-10
93/100 stars
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Construct:Article Title: Validation and analysis of 12,000 AI-driven CAR-T designs in the Bits to Binders competition. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Primary human T cells isolated from healthy-donor leukopaks by buoyancy-activated cell sorting (BACS) Sanguine Biosciences; Akadeum Life Sciences © The Author(s) Molecular Systems Biology 11 Reagent/resource Reference or source Identifier or catalog number Raji-GFP cells (Homo sapiens; CD20-positive target cells) Walcheck Lab at UMN CCL-86 K562-GFP cells (Homo sapiens; CD20-negative control target cells) ATCC CCL-234, GFP+ made at LEAH Recombinant DNA 12,000-variant second-generation CAR28z plasmid library (CD8alpha leader-80-aa binder-Whitlow 218 linker-CD28 hinge/ transmembrane and co-stimulatory domains-CD3zetaP2A-DHFR F31S) This study; oligo-pool cloning by Twist Bioscience; backbone modified from Van Der Stegen et al, 2015 Design sequences/data: https://doi.org/10.5281/ zenodo.20451404 Binderless secondgeneration CAR28z GeneWeld donor plasmid with TRAC homology arms This study; Webber et al, 2023; Wierson et al, 2020 Synthesized the CAR and cloned into a legacy vector system from Karl Clark. .. F31S dihydrofolate reductase methotrexateselection cassette Watanabe et al, 2015; incorporated into the CAR donor construct in this study As described in the CAR donor construct Binder-GFP11TwinStrep constructs for cellfree protein synthesis This study; Gene Fragments from Twist Bioscience Binder sequences/data: https://doi.org/10.5281/ zenodo.20451404 Control scFv CAR and cell-free expression constructs This study 1E4 antibody originally, https://patents.google.com/ patent/WO2017011316A1/en Antibodies Anti-Whitlow 218 linker-AlexaFluor 488 antibody (anti218L-AF488; 1:50) Cell Signaling Technology Clone: E3U7Q / catalog no. 55809S Anti-human TCR alpha/beta-APC antibody (1:50) Miltenyi Biotec Clone: REA652 / catalog no. 130- 113-535 Anti-human IL2-PE REA antibody (1:50) Miltenyi Biotec Clone: REA689 / catalog no. 130- Control:Article Title: Validation and analysis of 12,000 AI-driven CAR-T designs in the Bits to Binders competition. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Primary human T cells isolated from healthy-donor leukopaks by buoyancy-activated cell sorting (BACS) Sanguine Biosciences; Akadeum Life Sciences © The Author(s) Molecular Systems Biology 11 Reagent/resource Reference or source Identifier or catalog number Raji-GFP cells (Homo sapiens; CD20-positive target cells) Walcheck Lab at UMN CCL-86 K562-GFP cells (Homo sapiens; CD20-negative control target cells) ATCC CCL-234, GFP+ made at LEAH Recombinant DNA 12,000-variant second-generation CAR28z plasmid library (CD8alpha leader-80-aa binder-Whitlow 218 linker-CD28 hinge/ transmembrane and co-stimulatory domains-CD3zetaP2A-DHFR F31S) This study; oligo-pool cloning by Twist Bioscience; backbone modified from Van Der Stegen et al, 2015 Design sequences/data: https://doi.org/10.5281/ zenodo.20451404 Binderless secondgeneration CAR28z GeneWeld donor plasmid with TRAC homology arms This study; Webber et al, 2023; Wierson et al, 2020 Synthesized the CAR and cloned into a legacy vector system from Karl Clark. .. F31S dihydrofolate reductase methotrexateselection cassette Watanabe et al, 2015; incorporated into the CAR donor construct in this study As described in the CAR donor construct Binder-GFP11TwinStrep constructs for cellfree protein synthesis This study; Gene Fragments from Twist Bioscience Binder sequences/data: https://doi.org/10.5281/ zenodo.20451404 Control scFv CAR and cell-free expression constructs This study 1E4 antibody originally, https://patents.google.com/ patent/WO2017011316A1/en Antibodies Anti-Whitlow 218 linker-AlexaFluor 488 antibody (anti218L-AF488; 1:50) Cell Signaling Technology Clone: E3U7Q / catalog no. 55809S Anti-human TCR alpha/beta-APC antibody (1:50) Miltenyi Biotec Clone: REA652 / catalog no. 130- 113-535 Anti-human IL2-PE REA antibody (1:50) Miltenyi Biotec Clone: REA689 / catalog no. 130- Expressing:Article Title: Validation and analysis of 12,000 AI-driven CAR-T designs in the Bits to Binders competition. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Primary human T cells isolated from healthy-donor leukopaks by buoyancy-activated cell sorting (BACS) Sanguine Biosciences; Akadeum Life Sciences © The Author(s) Molecular Systems Biology 11 Reagent/resource Reference or source Identifier or catalog number Raji-GFP cells (Homo sapiens; CD20-positive target cells) Walcheck Lab at UMN CCL-86 K562-GFP cells (Homo sapiens; CD20-negative control target cells) ATCC CCL-234, GFP+ made at LEAH Recombinant DNA 12,000-variant second-generation CAR28z plasmid library (CD8alpha leader-80-aa binder-Whitlow 218 linker-CD28 hinge/ transmembrane and co-stimulatory domains-CD3zetaP2A-DHFR F31S) This study; oligo-pool cloning by Twist Bioscience; backbone modified from Van Der Stegen et al, 2015 Design sequences/data: https://doi.org/10.5281/ zenodo.20451404 Binderless secondgeneration CAR28z GeneWeld donor plasmid with TRAC homology arms This study; Webber et al, 2023; Wierson et al, 2020 Synthesized the CAR and cloned into a legacy vector system from Karl Clark. .. F31S dihydrofolate reductase methotrexateselection cassette Watanabe et al, 2015; incorporated into the CAR donor construct in this study As described in the CAR donor construct Binder-GFP11TwinStrep constructs for cellfree protein synthesis This study; Gene Fragments from Twist Bioscience Binder sequences/data: https://doi.org/10.5281/ zenodo.20451404 Control scFv CAR and cell-free expression constructs This study 1E4 antibody originally, https://patents.google.com/ patent/WO2017011316A1/en Antibodies Anti-Whitlow 218 linker-AlexaFluor 488 antibody (anti218L-AF488; 1:50) Cell Signaling Technology Clone: E3U7Q / catalog no. 55809S Anti-human TCR alpha/beta-APC antibody (1:50) Miltenyi Biotec Clone: REA652 / catalog no. 130- 113-535 Anti-human IL2-PE REA antibody (1:50) Miltenyi Biotec Clone: REA689 / catalog no. 130- Cell-Signaling:Article Title: Validation and analysis of 12,000 AI-driven CAR-T designs in the Bits to Binders competition. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Primary human T cells isolated from healthy-donor leukopaks by buoyancy-activated cell sorting (BACS) Sanguine Biosciences; Akadeum Life Sciences © The Author(s) Molecular Systems Biology 11 Reagent/resource Reference or source Identifier or catalog number Raji-GFP cells (Homo sapiens; CD20-positive target cells) Walcheck Lab at UMN CCL-86 K562-GFP cells (Homo sapiens; CD20-negative control target cells) ATCC CCL-234, GFP+ made at LEAH Recombinant DNA 12,000-variant second-generation CAR28z plasmid library (CD8alpha leader-80-aa binder-Whitlow 218 linker-CD28 hinge/ transmembrane and co-stimulatory domains-CD3zetaP2A-DHFR F31S) This study; oligo-pool cloning by Twist Bioscience; backbone modified from Van Der Stegen et al, 2015 Design sequences/data: https://doi.org/10.5281/ zenodo.20451404 Binderless secondgeneration CAR28z GeneWeld donor plasmid with TRAC homology arms This study; Webber et al, 2023; Wierson et al, 2020 Synthesized the CAR and cloned into a legacy vector system from Karl Clark. .. F31S dihydrofolate reductase methotrexateselection cassette Watanabe et al, 2015; incorporated into the CAR donor construct in this study As described in the CAR donor construct Binder-GFP11TwinStrep constructs for cellfree protein synthesis This study; Gene Fragments from Twist Bioscience Binder sequences/data: https://doi.org/10.5281/ zenodo.20451404 Control scFv CAR and cell-free expression constructs This study 1E4 antibody originally, https://patents.google.com/ patent/WO2017011316A1/en Antibodies Anti-Whitlow 218 linker-AlexaFluor 488 antibody (anti218L-AF488; 1:50) Cell Signaling Technology Clone: E3U7Q / catalog no. 55809S Anti-human TCR alpha/beta-APC antibody (1:50) Miltenyi Biotec Clone: REA652 / catalog no. 130- 113-535 Anti-human IL2-PE REA antibody (1:50) Miltenyi Biotec Clone: REA689 / catalog no. 130- FACS:Article Title: Single-cell transcriptomic reference map of human peripheral blood γδ T cells from 1 year to 90 years reveals major changes before adulthood. Article Snippet: Following surface staining, cells were fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) according to the manufacturer’s protocol, and stained intra- cellularly with anti-Perforin BV421 (dG9, BioLegend), anti-GZMK PerCP-eFluor710 (G3H69, Invitrogen), and anti-GZMB AF647 (GB11, BioLegend). .. FACS sorting of CD28+ Vδ2 T cells and HMBPP stimulation PBMCs were thawed, counted, and ∼2 × 107 cells per donor were stained for 15 min at room temperature with anti-CD3 AF488 (UCHT1, BioLegend), anti-γδ TCR PE (REA591, Miltenyi Biotec), anti-CD28 PE-Cy5 (CD28.2, BioLegend), and Staining:Article Title: Single-cell transcriptomic reference map of human peripheral blood γδ T cells from 1 year to 90 years reveals major changes before adulthood. Article Snippet: Following surface staining, cells were fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) according to the manufacturer’s protocol, and stained intra- cellularly with anti-Perforin BV421 (dG9, BioLegend), anti-GZMK PerCP-eFluor710 (G3H69, Invitrogen), and anti-GZMB AF647 (GB11, BioLegend). .. FACS sorting of CD28+ Vδ2 T cells and HMBPP stimulation PBMCs were thawed, counted, and ∼2 × 107 cells per donor were stained for 15 min at room temperature with anti-CD3 AF488 (UCHT1, BioLegend), anti-γδ TCR PE (REA591, Miltenyi Biotec), anti-CD28 PE-Cy5 (CD28.2, BioLegend), and Article Title: In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans Article Snippet: .. Cells were stained with combinations of the following antibodies for 20 min at 4 °C: anti‐CD3‐BV650 (clone UCHT1, BD Pharmigen, San Diego, CA, USA), anti‐CD3‐PECF594 (clone SP34‐2, BD biosciences, San Jose, CA, USA), Article Title: Pharmacologic HIF-2α inhibition preserves T cell effector function in clear cell renal cell carcinoma Article Snippet: Cells were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences, catalog #554714) according to the manufacturer's instructions. .. Intracellular cytokine staining was performed using anti-IFN-γ-BV711 (BioLegend, catalog #502539), Incubation:Article Title: Unveiling the gut microbiome-immune-brain axis in schizophrenia Article Snippet: .. For that, 50 μL of whole blood was incubated with 1μL of each antibody: CD45 (Miltenyi Biotec, Clone REA737, Cat. No. 130-110-665), CD3-FITC (Miltenyi Biotec, Clone REA641, Cat. No. 130-131-559), Tissue:Article Title: Ultrasensitive Trace Sample Proteomics for Pancreatic Ductal Adenocarcinoma Diagnosis Empowered by Dual-Affinity Spherical Ln-MOFs. Article Snippet: Low-abundance exosomal proteins from trace plasma volumes remain challenging to profile by mass spectrometry.. Here, we report a lanthanide metal–organic framework (Ln-MOF) functionalized with boronic acid and carboxylate dual ligands, which captures exosomal proteins via synergistic phosphate coordination, boronate-diol complexation, and cationπ interactions.. Compared with traditional ultracentrifugation, which identifies 1003 proteins from 200 μL of plasma, the Ln-MOF method identifies 1523 proteins from only 50 μL of plasma. Saline:Article Title: Ultrasensitive Trace Sample Proteomics for Pancreatic Ductal Adenocarcinoma Diagnosis Empowered by Dual-Affinity Spherical Ln-MOFs. Article Snippet: Low-abundance exosomal proteins from trace plasma volumes remain challenging to profile by mass spectrometry.. Here, we report a lanthanide metal–organic framework (Ln-MOF) functionalized with boronic acid and carboxylate dual ligands, which captures exosomal proteins via synergistic phosphate coordination, boronate-diol complexation, and cationπ interactions.. Compared with traditional ultracentrifugation, which identifies 1003 proteins from 200 μL of plasma, the Ln-MOF method identifies 1523 proteins from only 50 μL of plasma. Magnetic Cell Separation:Article Title: Ultrasensitive Trace Sample Proteomics for Pancreatic Ductal Adenocarcinoma Diagnosis Empowered by Dual-Affinity Spherical Ln-MOFs. Article Snippet: Low-abundance exosomal proteins from trace plasma volumes remain challenging to profile by mass spectrometry.. Here, we report a lanthanide metal–organic framework (Ln-MOF) functionalized with boronic acid and carboxylate dual ligands, which captures exosomal proteins via synergistic phosphate coordination, boronate-diol complexation, and cationπ interactions.. Compared with traditional ultracentrifugation, which identifies 1003 proteins from 200 μL of plasma, the Ln-MOF method identifies 1523 proteins from only 50 μL of plasma. Analysis:Article Title: Ultrasensitive Trace Sample Proteomics for Pancreatic Ductal Adenocarcinoma Diagnosis Empowered by Dual-Affinity Spherical Ln-MOFs. Article Snippet: Low-abundance exosomal proteins from trace plasma volumes remain challenging to profile by mass spectrometry.. Here, we report a lanthanide metal–organic framework (Ln-MOF) functionalized with boronic acid and carboxylate dual ligands, which captures exosomal proteins via synergistic phosphate coordination, boronate-diol complexation, and cationπ interactions.. Compared with traditional ultracentrifugation, which identifies 1003 proteins from 200 μL of plasma, the Ln-MOF method identifies 1523 proteins from only 50 μL of plasma. |